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Fig. 2. Expression of Gb'hb during embryogenesis. Expression patterns of Gb'hb at 30 (A,B), 32 (C), 36 (D), 40 (E), 42 (F), 48 (G), 52 (H,I) and 72 (J,K) hAEL. The embryo was double-stained with Gb'wg in B (Gb'hb, blue; Gb'wg, brown), E (Gb'hb, brown; Gb'wg, blue) and K (Gb'hb, blue; Gb'wg, brown). (C,D) Stripes of Gb'hb expression in the middle gap domain are indicated by arrowheads. The posterior expression domain (arrowheads in G,H,J) appeared at 48 hAEL (G) and split into two stripes (J). (K) Double staining for Gb'hb (blue) and Gb'wg (brown) indicates that the splitting intense domain of the Gb'hb expression near the posterior end spanned from the A7 stripe to the A9 stripe of Gb'wg. (I) High-magnification view of the boxed area in H, showing spotted expressions. Mn, mandible; Mx, maxilla; Lb, labium; T1-3, thoracic segments 1 to 3; A7-9, abdominal segments 7 to 9. Scale bars: 250 µm in A-H,J; 100 µm in K.





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