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Fig. 3. Effect of Gb'hb RNAi on G. bimaculatus embryos. Anterior
towards the left. (A,B) Wild-type embryo in the stage just before hatching.
(A) Lateral view. (B) Dorsal view. (C) Wild-type embryo stained for
Gb'wg. (D,E) Class III embryo. (D) Lateral view. (E) Dorsal view. (F)
Putative class III embryo stained for Gb'wg. In this embryo,
segmentation of the T1 to T3 was partially disturbed (arrow). Segmentation
defects were frequently observed in Gb'hb RNAi embryos (also shown in
H and I). (G,H) Class II embryo. (G) Lateral view. Arrowhead indicates a
defective T3 leg. (H) Dorsal view. (I) Putative class II embryo stained for
Gb'wg. Arrows in H and I indicate segmentation disturbances. (J-L)
Class I embryo. (J) Lateral view. Arrowhead indicates the mandible. (K) Dorsal
view. (L) Ventral view. Arrowhead indicates a vestige of a T3 leg. (M)
Putative class I embryo stained for Gb'wg. (N-S) Sagittal sections
through wild-type and Gb'hb RNAi embryos. (N,Q) Wild type. (O,R)
Class II embryo. (P,S) Class I embryo. Each of these pairs was derived from
the same specimen. The digestive tract consists of a fore-, mid- and hindgut.
(N) In wild type, the pharynx (ph), esophagus (es), crop (cr) and
proventriculus (pv) are observed in the foregut. The midgut contains yolk
granules. (O) In class II embryos, the fore- and hindgut were compressed and
the midgut was relatively shifted towards the anterior. (P) In more severe
cases, a sac-like midgut was observed, and the fore- and hindgut were further
compressed. (Q) The musculature in the abdominal segments shows a reiterated
pattern (arrowheads). (R,S) In Gb'hb RNAi embryos, musculature was
observed in each segment, although the reiterated pattern was not orderly.
Arrows in P and R indicate a vestige of the T3 leg. an, antenna; cr, crop; es,
esophagus; fg, foregut; ph, pharynx; mo, mouth; pv, proventriculus; mg,
midgut; hg, hindgut; T, thorax; Mn, mandible; Mx, maxilla; Lb, labium; T1-3,
thoracic segments 1 to 3. Scale bars: in A, 400 µm for A,B,D,E,G,H,J,K,L;
in N, 400 µm for N-S; 200 µm in C,F,I,M.
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