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Fig. 6. In situ detection of segmentation and Hox genes in early-stage embryos
depleted of Gb'hb by RNAi. (A-C) Expression patterns of
Gb'Kr in wild-type (A) and Gb'hb RNAi embryos (B,C) at 40-42
hAEL. Gb'Kr is expressed in the gnathal and thoracic regions at this
stage in wild-type embryos. Expression in this domain was reduced by
Gb'hb depletion and, in severe cases, almost eliminated. The embryo
shown in C was affected more severely than that shown in B. Gb'hb
RNAi embryos are shorter and flatter than the wild type. (D-F) Expression
patterns of Gb'eve in wild-type (D) and Gb'hb RNAi embryos
(E,F) at 38-40 hAEL. In wild-type embryos at this stage, Gb'eve is
expressed as five clearly resolved stripes and an additional one or two
stripes are resolving from the posterior broad domain. (E) In Gb'hb
RNAi embryos, the number of stripes was reduced to less than five. (F) In more
severe cases, only three stripes were observed. (G-I) Expression patterns of
Gb'wg in wild-type (G) and Gb'hb RNAi embryos (H,I) at 45-47
hAEL. (G) At this stage of wild type, seven Gb'wg stripes are
observed in the gnathal and thoracic regions. (H) In Gb'hb RNAi
embryos, the stripes in the thoracic region were affected in that they had an
irregular shape and the number of stripes was reduced from three to two. (I)
In more severe cases, the labial stripe was also disturbed. (J,K) Expression
patterns of Gb'Antp (brackets) in wild type (J) and a Gb'hb
RNAi embryo (K) at 38-40 hAEL. (K) The Gb'Antp domain was severely
reduced in Gb'hb RNAi embryos. (L,M) Expression patterns of
Gb'abdA in wild type (L) and a Gb'hb RNAi embryo (M) at
45-47 hAEL. (L) In wild type, the Gb'abdA expression in the abdominal
region begins close to this stage. (M) In Gb'hb RNAi embryos, the
ectopic Gb'abdA expression was observed in the gnathal and thoracic
regions. Scale bar: 250 µm.
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