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Fig. 1. Cdx2 is expressed in cells of the TE lineage. (A) DIC image of late morula
stage embryo (
16 cell). (B) Immunofluorescent localization of Cdx2 in
embryo shown in A. Monoclonal anti-Cdx2 antibody detects high levels of Cdx2
in nuclei of outer cells (arrow) and lower cytoplasmic levels (arrowhead). (C)
Polyclonal anti-Cdx2 (CNL) antibody detects similar expression pattern,
including cytoplasmic staining (arrowhead) at late morula (25 cell)
stage. (D) Oct4 and Cdx2 protein in same embryo shown in C. Cdx2 (red) is
restricted to outer cells, but Oct4 (green) is still detectable in all outer
cells. Oct4 and Cdx2 colocalize in outer cells (e.g. black arrowhead). (E-I)
Composite DIC confocal images of polyclonal anti-Cdx2 staining of (E) 3.25 dpc
early blastocyst, (F) 3.5 dpc blastocyst, (G) 4.5 dpc implanting blastocyst,
(H) 4.75 dpc and (I) 5.5 dpc. Arrow indicates nuclear staining, white
arrowhead indicates cytoplasmic. Red arrowheads demarcate polar TE. Bracket
indicates ExE. (J) DIC image of Cdx2-/- morula. (K)
Monoclonal anti-Cdx2 staining of embryo shown in J. (L) DIC image of
Cdx2-/- blastocyst. (M) Monoclonal anti-Cdx2 staining of
embryo shown in L. (N) DIC image of wild-type blastocyst. (O) Monoclonal
anti-Cdx2 staining of embryo shown in N. Scale bar: 15 µm in A-G; 30 µm
in H,I; 20 µm J-O.
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