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Fig. 6. Nanog is not downregulated in the outer cells of Cdx2-/- embryos. (A) Immunolocalization of Nanog in Cdx2+/+ morula. (B) Nanog in Cdx2-/- morula, littermate of embryo shown in A. Nanog is detected in inner and outer cells in non-mutant and mutant morulae. (C) Nanog in Cdx2+/+ in early blastocyst. Low levels of Nanog can be detected in occasional TE nuclei (arrowhead), but is largely downregulated in the TE by this stage. (D) Nanog in Cdx2-/- early blastocyst. Nanog expression persists in TE cells in Cdx2 mutants. All panels are projections of multiple optical sections.





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