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Fig. 7. Cultured Cdx2-/- embryos exhibit excessive cell death
at the expanded blastocyst stage. (A-B') Composite images of single
confocal optical sections superimposed on DIC images. Zona pellucida-free
eight-cell stage embryos from Cdx2+/– intercrosses
were cultured for 48 hours followed by TUNEL analysis. (A)
Cdx2+/–; TUNEL-positive nuclei were detected in the
ICM. (B,B') Cdx2-/- blastocyst; TUNEL-positive
nuclei were observed in the ICM and TE. (B') Optical section taken at a
different level of the embryo in B. Scale bars: 25 µm. (C) The number of
TUNEL-positive nuclei in the ICM and TE of Cdx2+/+ or
Cdx2+/– embryos (n=16) and
Cdx2-/- embryos (n=7) was scored from confocal
optical sections of individual embryos following TUNEL staining. The values
plotted represent the mean number of TUNEL-positive nuclei (±s.e.m.).
The increase in the number of TUNEL positive nuclei in the ICM and TE in
Cdx2-/- embryos compared with
Cdx2+/– or Cdx2+/+ embryos was
significant at P<0.001 using Student's t-test
analysis.
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