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Fig. 10. Implantation of GDF5 (B,E,H) and BMP4 (C,F,J) beads (*) into the distal regions of E11.0-12.5 limbs results in ectopic expression of Barx2. mRNA and protein expression were visualized using in situ hybridization (A-C,G,J) and immunohistochemistry (D-F,H), respectively. Nuclei were stained with Oli-green. Arrows indicate ectopic Barx2 expression. Black arrows indicate endogenous Barx2 expression; white arrows indicate ectopic Barx2 expression; en, endogenous expression.





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