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Fig. 7. Schematic of the intronic regulatory region of the Col2a1 gene.
(A) Two overlapping segments of 468 and 309 bp have previously been shown to
drive chondrocyte specific expression in cell cultures and in transgenic mice
(Bell et al., 1997;
Zhou et al., 1995). These
regions both include a 48 bp element containing three HMG-binding sites (HMG1,
HMG2 and HMG3). Two additional HMG motifs (HMG4, HMG5) were identified
downstream of this element. Two conserved putative homeodomain binding sites
(designated HBS1 and HBS2) were identified by comparison of human and mouse
genes. Boxes indicate the sequences used as probes in this study. (B)
Sequences of wild-type and mutant probes. The core of binding motifs
containing the sequence ATTA is indicated in bold. Mutations are indicated in
lower case. (C) Western blot analysis shows that Barx2 and Sox9 are expressed
in E12.5 limb nuclear extract.
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