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Fig. 3. p38 is required for oral specification at late blastula stage.
(A) Schematic depicting the experiment in B. Corresponding hours
post-fertilization (h) are indicated. (B) Embryos were treated with SB
at the indicated stages (B1-B4), and then cultured to the pluteus stage.
Morphological (top) and skeletal (bottom, illuminated with plane-polarized
light) images are shown. (C-E) Whole-mount in situ analysis for Nodal
(C), Gsc (D) and Tbx2/3 (E) in embryos treated with SB at 5 (1), 6 (2,3) and 7
(4) hpf. Vegetal views at late gastrula stage are shown. EB, early blastula;
HB, hatched blastula; TVP, thickened vegetal plate; MB, mesenchyme blastula;
Plut, pluteus larvae.
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