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Fig. 6. Nodal expression oralizes embryos downstream from p38. (A) A1 and A2 show DIC images of a control embryo (A1) and an embryo injected with Nod MASO (0.7 mM, A2) at the pluteus stage. (A3) Embryo co-injected with Nod MASO and Lvp38-GFP, then imaged with live confocal microscopy at late blastula stage, as in Fig. 5C and 5E. (B-E) Whole-mount in situ analysis of Gsc (B), Dri (C), Tbx2/3 (D) and Bra (E) expression shown in control (1), SB-treated (2), Nod-injected (3), and Nod-injected plus SB-treated (4) embryos. Vegetal views are shown at prism stage, except for D (lateral views; D1 at late gastrula and D2-D4 at pluteus stage).





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