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Fig. 1. pMAPK antigen, MAPK/GAL4 (MG)-driven GFP, DNA and
bs:lacZ expression in wild-type larval wings. Anterior
upwards, dorsal leftwards. Magnifications are equal in A and F, C and D, and E
and G. (A) pMAPK is in red, MG-driven GFP is in green. (B)
Confocal section of large boxed area at arrow in A. pMAPK is in red, MG-driven
GFP is in green. MG-driven GFP is absent from margin cells (arrowheads). An
example of a MG-driven GFP responsive nuclei is indicated by an arrow.
(C,D) A single confocal section of vein tissue (C) through small boxed
area at arrowhead in A, and lateral cross-section of margin tissue (D) at
boxed area in B. pMAPK in red, DNA in blue. pMAPK is predominantly cytoplasmic
in both regions (arrowhead indicates pMAPK staining next to pMAPK-negative
nucleus at arrow). Bracket in D separates two columns of pMAPK stains at wing
margin (arrowheads in B). (E) Confocal section in the wing pouch of the
wing margin and veins L3, L4 and L5. pMAPK is in red, MG-driven GFP is in
green, actin is in blue. Cells positive for pMAPK (arrows) and GFP
(arrowheads) are indicated. (F,G) bs:lacZ is in red, MG-driven
GFP is in green. GFP-positive nuclei devoid of lacZ (arrow in G), and
GFP and lacZ nuclei are indicated (arrowhead in G). Broken white
lines define area of bs:lacZ expression in G.
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