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Fig. 2. pMAPK antigen, MAPK/GAL4 (MG)-driven GFP and DNA expression in wild-type
pupal wings. Anterior is upwards, distal is rightwards. Hours APF is
indicated in the upper right-hand corner. pMAPK is in red, MG-driven GFP is in
green, DNA is in blue in all panels. (A) pMAPK antigen is predominant
in pupal veins (asterisk). (B) At 28 hours APF, pMAPK expression falls
in the veins (compare asterisks in A and B). (C) High magnification of
distal wing margin at arrowhead in B. MG-driven GFP is present in two lines of
cells flanking pro-veins (arrows). (D) pMAPK returns to veins (compare
asterisks in B and D). (E) High magnification of boxed area at arrow in
A. (F) High magnification of boxed area at arrow in B. pMAPK is
predominantly cytoplasmic in both E and F; arrowheads indicate pMAPK staining
next to pMAPK-negative nuclei at arrows.
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