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Fig. 3. Overcoming MAPK cytoplasmic hold in the developing wing. Stages are indicated on the left. Genotypes indicated. (A-D) Adult wings after a 1-hour heat induction at 20-24 APF (A,B) and 24-27 APF (C,D). (E,F) Bar graphs showing comparison of wing-surface areas (E) and cell counts/hair density (F) between genotypes depicted in A-D. The hs:rho; hs:msk wing area is larger in E; P<10^–7 in each case. hs:NM wings contain more cells in F (P<10^–4 in each case). (G-K) Twenty-four hour APF pupal wings (anterior leftwards, distal upwards), pMAPK antigen white. (H-K) One-hour heat induction, then recovery for the time indicated top right in hours. Magnifications are equal in A-D and G-K. Elevated pMAPK at asterisks in H and I compared with G, J and K. P values in E are: hs:M to hs:NM=0.3, hs:M to hs:rho=0.6, hs:M to hs:rho; hs:msk=4.4x10^–8, hs:NM to hs:rho=0.8, hs:NM to hs:rho; hs:msk=9.1x10^–9, and hs:rho to hs:rho; hs:msk=5.3x10^–7. P values in F are: hs:M to hs:NM=1.3x10^–4, hs:M to hs:rho=0.03, hs:M to hs:rho; hs:msk=0.1, hs:NM to hs:rho=1.0x10^–5, hs:NM to hs:rho; hs:msk=1.5x10^–5 and hs:rho to hs:rho; hs:msk=0.2.

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