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Fig. 5. Osteoprogenitor proliferation at the interfrontal and coronal sutures of
wild-type and En1-/- embryos between E12.5-E18.5.
(A) Immunohistochemical detection of BrdU-labeled cells within the
interfrontal sutures (dashed outline). (B) Proliferation was
numerically assessed as the fraction of labeled to non-labeled cells. A
specific reduction in osteoprogenitor proliferation was observed in
En1-/- calvariae at E18.5. (C) Enhanced
osteoprogenitor proliferation within the coronal suture (arrowheads) of
En1 mutants at E18.5. p, parietal bone; f, frontal
bone. Scale bar: 0.2 mm.
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