|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Increased remodeling and osteoclast recruitment in
En1-/- calvariae. (A) Alizarin Red stained
wild-type and En1-/- calvariae at P1 showing multiple
perforations in the mutants (arrows). (B) Whole-mount detection of
osteoclast-specific TRAP activity in calvariae at P5, depicting increased
staining in En1 mutants (compare with wild type, arrow). (C)
Histological detection of TRAP+ osteoclasts located along the
endocranial (arrows) and trabecular (arrowheads) bone surfaces of the frontal
bones at P1. (D) Quantitative histomorphometric assessment of
osteoclast number in sections of wild-type and En1-/-
frontal bones. (E,F) Northern blot and semi-quantitative analysis of
OPG and Rankl expression in cultured calvarial osteoblasts.
At day 7 of postconfluent growth, wild-type and En1-/-
cells express similar levels of OPG, whereas expression of
Rankl is strongly upregulated in mutant osteoblasts. F, frontal bone;
P, parietal bone. Scale bars: 2 mm in A,B; 0.1 mm in C.
|
