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Figure 7


Fig. 7. Increased Hippo signaling, but not cell competition, controls apoptosis in vps25 clones. (A-C) Caspase-3*-labeling of vps25 mosaics (genotype: ey-FLP; FRT42D vps25N55/FRT42D arm-lacZ M(2)) in a Minute (M) background. Caspase-3* activity is unaffected. Clones are marked by the absence of ß-Gal staining. (D-F) Caspase-3*-labeling of GFP-marked vps25/dMyc mosaics (hs-FLP UAS-GFP; FRT42 vps25N55/FRT42D tub-Gal80; tub-Gal4/UAS-dMyc). Caspase-3*-activity is unaffected. (G-I) Expanded (Ex) labeling of GFP-marked vps25 mosaics (hs-FLP UAS-GFP; FRT42D vps25N55/FRT42D tub-Gal80; tub-Gal4). Ex protein levels are reduced in vps25 clones (arrow), indicating increased Hippo activity. (J-L) Caspase-3*-labeling of GFP-marked vps25 hippo double mutant mosaics (hs-FLP UAS-GFP; FRT42D vps25N55 hippo3D/FRT42D tub-Gal80; tub-Gal4). Caspase-3* activity is blocked.





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