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Figure 2


Fig. 2. smp expression pattern throughout medaka embryonic development. WMISH at different embryonic stages. (A,B) Maternal smp transcripts were detected at the one-cell stage (A) and the four-cell stage (B). (C,D) At the 512-cell stage, transcripts were detected in central blastomeres but not in peripheral ones (D, arrow). (E,F) Transverse sections confirm that smp expression is not present in peripheral blastomeres (arrow), and that Ol-pou5f1 is expressed in both inner and marginal ones. (G,H) From 2-somite stage to 16-somite stage, smp is expressed at the anterior midline of the telencephalon (t), at the prospective midbrain-hindbrain boundary (mhb) and in the recently formed somites (s). (I) In 35-somite embryos, smp is detected in the progenitor region of the retina (r, arrow) and in a large portion of the CNS, including the forebrain (f), optic tectum (ot), mhb and rhombic lips (rl).





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