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Figure 3


Fig. 3. Overexpression of DN cadherin constructs but not full-length cadherin constructs slows down the migration of LRN/ECN neurons. Flat whole-mount preparations of rat hindbrains electroporated with expression vectors encoding EGFP (A), EGFP+CAD11 (B), EGFP+NCAD (C), EGFP+cN390{Delta} (D) and EGFP+EGFP-NCAD(t) (E) at 3 div. (Left column) Lower magnification views of flat whole-mount preparations in which migrating neurons are labeled with EGFP. (Middle and right columns) Higher magnification views of contralateral and ipsilateral sides, respectively, in the flat whole-mount preparations in the left column. Many migrating neurons with EGFP, EGFP+CAD11 and EGFP+NCAD that have crossed the ventral midline are seen on the contralateral side (middle column in A-C). By contrast, fewer cells with EGFP+cN390{Delta} and EGFP+EGFP-NCAD(t) are observed in the contralateral part, apart from the ventral midline (middle column in D and E). Denser cell clumps are seen on the ipsilateral side of the preparations with DN cadherins (right column in D and E) compared with the control preparations with only EGFP or the preparations with exogenous full-length cadherins (right column in A-C). Broken lines labeled VM indicate the position of the ventral midline. Scale bars: 400 µm in left column; 50 µm in middle and right columns.





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