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Fig. 6. Overexpression of a DN cadherin construct disrupts the migration of
LRN/ECN neurons in vivo. E15.5 mouse hindbrains electroporated with Venus
YFP (A,C,E,G) and EGFP-NCAD(t) (B,D,F,H) at E12.5. Lower magnification views
from dorsal (A,B) and ventral (C,D) side. The
right lower rhombic lip is exclusively labeled with fluorescent markers. PEMS
and AEMS corresponding to the migratory streams of LRN/ECN neurons and of
pontine gray and reticulotegmental nuclei neurons, respectively, are seen in
the ventral views. Rostral is towards the left. Asterisks represent the
labeled side. Cb, cerebellum; AEMS, anterior extramural migratory stream;
PEMS, posterior extramural migratory stream. Higher magnification views of
ipsilateral (E,F) and contralateral (G,H) sides.
More control neurons are seen on the contralateral side in controls, whereas
more neurons expressing EGFP-NCAD(t) are found on the ipsilateral side. Broken
lines labeled VM indicate the position of the ventral midline. Scale bars: 1
mm in A-D; 200 µm in E-H.
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