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Figure 5


Fig. 5. Pax7EnR functions as a dominant-negative form of Pax7. (A,C,D,F) Embryos injected with 200 pg pax7EnR mRNA and 80 pg GFP into left side of dorsal animal hemisphere at four cell stage. (A) A stage16 embryo; the arrow indicates the anterior neural fold defect on left-hand side. (C) Left side of pax7EnR injected embryo, the arrow indicates the developing eye. The fluorescence in the inset shows a small lens underneath the epidermis. (D) Eye development on the un-injected side. (F) A tadpole with a smaller left eye (arrows). (B,E) Co-injection of 500 pg pax7 mRNA, 200 pg pax7EnR mRNA and 80 pg GFP is able to rescue the defective eyes to normal size. (G) Injection of 500 pg pax7 and 80 pg gfp mRNA into one side of the dorsal animal hemisphere results in an ectopic eye in the forebrain of stage 43 tadpoles. The GFP fluorescence in the insets indicates the injected region. (H-J) Transverse sections through ectopic eye of injected tadpole (showed in inset) were stained with Hematoxylin and Eosin. The arrows in G,H indicate the extra eyes. (I) A magnified view of the ectopic eye and one normal eye. The asterisk in J highlights the tube protruding from the ventral midbrain. Scale bars: 300 µm.





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