DEV02435 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Skeletal cell fates of wild-type and moz^- CNC. Lateral (A,D,G,J,M,P,S) and dorsal cross-sectional (B,E,H,K,N,Q,T) views of labelled CNC at 24 hpf and their resultant positions in the facial skeleton at 4 dpf (C,F,I,L,O,R,U). Wild-type examples include CNC precursors of second-segment Ch (A-C) and posterior Hm (D-F) cartilages, second-segment Br osteocytes (G-I), and first-segment Pq (J-L) and M (M-O) cartilages. moz^- examples include second-segment CNC precursors of Pq’ (P-R) and M’ (S-U) cartilages. In a, the outline of medial pharyngeal endoderm (Mpe) is shown. In general, precursors of the distal region of Ch are directly adjacent to medial endoderm, whereas precursors of proximal Ch map to more lateral positions. Scale bars: 50 μm.

• Movie 1 -

  Movie 1. Facial skeletal development in a wild-type fli1:GFP animal with mosaic labelled CNC (red).

• Movie 2 -

  Movie 2. Facial skeletal development in a moz^-; fli1:GFP animal.

• Movie 3 -

  Movie 3. Mosaic wild-type CNC (red) cell-autonomously form cartilage nodules in a moz^-; fli1:GFP host.