DEV02444 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Alignment of ISW-1 with Drosophila ISWI and S. cerevisiae SWI2/SNF2. Solid boxes indicate two out of three amino acids are identical in ISW-1, ISWI and SWI2/SNF2. Red arrowheads indicate sites of amino acid changes caused by the isw-1 missense mutations. The isw-1(n4066) deletion allele is marked by blue boxes with arrows pointed towards the deleted region.  The AT-hook, ATPase, DEAH/D box helicase and SANT domains are indicated by a black and white box, by a dark-gray box, by a diagonally hatched box and by black boxes, respectively.

• Supplemental Figure 2 -

  Fig. S2. ISW-1 is broadly expressed in the nuclei of most if not all cells throughout development. (A) Anti-ISW-1 antisera are specific for ISW-1. A western blot showing the specificity of the ISW-1 antisera by the presence of a ~110 kDa band in the wild-type protein samples from 50 and 25 animals (lanes 1 and 2) but not in the isw‑1(n4066) mutant protein samples from 50 and 25 animals (lanes 3 and 4). For B-E, red is anti-ISW-1 staining and blue is DAPI. The colocalization appears as purple. From top to bottom: DAPI, anti-ISW-1 and merge. (B) ISW-1 expression in a young adult. Scale bar: 100 μm. (C) ISW-1 expression in a ~64-cell embryo. Scale bar: 10 μm. (D) ISW-1 expression in third stage larva. Scale bar: 100 μm. (E) ISW-1 expression in an adult germline. ISW-1 staining is high in the oocytes (white arrows). Scale bar: 50 μm.

• Supplemental Figure 3 -

  Fig. S3. Alignment of the predicted domains of NURF-1 and Drosophila NURF301. Solid boxes indicate identity between NURF-1 and NURF301. The domains are shown from N terminus to C terminus and correspond to the domains shown in Fig. 2B. (A) AT-hook motifs of the HMGA domain; (B) DDT domains; (C) PHD fingers; (D) bromodomains.

• Supplemental Figure 4 -

  Fig. S4. Shown are the mean levels of GFP expression from the ccIs4251 transgene reporter in the experiments shown in Figure 4D, as measured by number of pixels in a defined region of interest from at least 10 animals in each genetic background. The fluorescence from the vulva to the posterior end was quantified within the linear range of detection (see Materials and methods). Error bars indicate s.d.