DEV02467 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Dfer protein expression. (A) Whole embryo extracts were probed with anti-Dfer N-terminal antibody. Several bands are non-specific (arrowhead). A band of the expected size for the canonical isoform of DFer (arrow) is reduced in dfer^Δex1 and absent in dfer^Δ1 mutants. (B) This band is present in dfer^gof mutants, accompanied by a second band corresponding to the wex1-DFerRB protein (arrows). (C,D) Loading controls (anti-actin).

• Supplemental Figure 2 -

  Fig. S2. Dfer is not a transcriptional target of the JNK pathway. (A-F) dpp (A-C) and dfer (D-F) expression in (A,D) wild type, (B,E) hep¹ mutants, and (C,F) embryos expressing an activated form of Hep in engrailed stripes. The JNK pathway target dpp is (B) lost in JNK pathway mutants and (C) ectopically activated by Hep^CA. By contrast, (E) dfer leading edge expression is present in hep¹ mutants and (F) dfer expression is not upregulated by Hep^CA.

• Supplemental Figure 3 -

  Fig. S3. Schematic diagram of Fer and Src function at adherens junctions. Vertebrate model of Fer and Src action at AJs. Fer kinase phosphorylates β-catenin at tyrosine residue 142, releasing α-catenin from the AJ complex. Src kinase phosphorylates β-catenin at tyrosine residue 654, releasing it from E-cadherin.