DEV02482 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Development of the tracheal system of a btlGa4 UAS-tauGFP; 801 embryo from stage 14, focused on the DT and DBs. As development proceeds, branch integrity defects in the DBs are clearly observed. Cells of one of the DBs (arrow) begin to pull apart giving rise to cells abnormally separated and only attached to the stalk by a thin cytoplasmic extension. Finally, a cluster of cells detaches from the branch. Note the presence of floating cells around the tracheal system. These are likely to be macrophages.

• Supplemental Figure 1 -

  Fig. S1. Histogram showing the quantifications of E-cadherin levels assayed with DCAD2 antibody. Each pair of bars represents the average level of the control population (pink) and the mutant population (green) in each independent experiment. (A) Measurements of the DBs; (B) measurements of the DT. n represents the number of embryos analysed