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Fig. 5. DE-cad and actin accumulation in the tracheal system are affected by the
Egfr pathway. (A-E') Projections of confocal sections showing a
portion of DT and two to three DBs of the posterior region of stage 16
embryos. The embryos are labelled with DCAD2 (red in A-E) to detect DE-cad and
anti-GFP (A,C-E) or anti-Trh (B) to detect the tracheal cells (green).
Greyscale images of DCAD2 are shown separately in panels A'-E'. Note that
under conditions of Egfr downregulation (B-D), the levels of DCAD2
are lower than in wild type (A), and the staining in the DBs (arrows) is less
conspicuous. By contrast, high levels are detected when Egfr is constitutively
activated (E), and some DBs remain multicellular, as visualised by the
presence of intercellular AJs (arrow). Note that DE-cad is properly localised.
(F-J') Single confocal sections showing details of the DT of the
posterior region of stage 16 embryos. The embryos are labelled with anti-actin
(red in F-J) and anti-GFP (green in G,I,J) or anti-Trh (green in F,H).
Greyscale images of anti-actin are shown alongside. Note the presence of a
thick cortical staining in the wild-type (F) compared with staining in
conditions of Egfr downregulation (G-I). A thicker accumulation of
cortical actin is detected when Egfr is constitutively activated (J).
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