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Fig. 7. Newly synthesized DE-cad in Egfr downregulation conditions. Late stage embryos labelled with mAb2A12 (red) and anti-GFP to detect the DE-cadGFP protein (green in A-E; greyscale in A'-E') under the control of heterologous promoters. (A-C') Projections of confocal sections of embryos carrying btlGal4 UAS-DE-cadGFP. The GFP signal in B and C is weaker and not as clearly detected in some thin tracheal branches (arrows) as in the wild type (A). (D,E,G,H) Single confocal sections showing tracheal branches (D,E) or salivary glands (G,H) of embryos carrying 69B UAS-DE-cadGFP. Note the lower levels of GFP staining in embryos overexpressing 801, especially in the tracheal branches (arrow in E'), and the reduced accumulation of the chimaeric protein in the apical part of the salivary glands (arrows in G,H). (F) Western blot analysis of GFP to detect the DE-cadGFP protein level and ${alpha}$ -tubulin (as a protein loading control) in embryos with indicated genotypes. After densitometric quantification of GFP and ${alpha}$ -tubulin levels, we detected a 30% decrease in GFP levels in line 801 (lane 5 compared with lane 3) and ${lambda}$ top showed a >3-fold increase (lane 1 compared with lane 3).

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