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Fig. 5. Morpholino co-injections reveal evidence of interactions between
san, vtn and heg. Injection of low doses (10
and 15 µmol/l) of each morpholino alone is unable to produce large number
of embryos with the characteristic enlarged heart and thin-walled myocardium.
However, by injection of combinations of any two of the san, vtn and
heg morpholinos, a dramatic increase in the level of phenocopy is
observed (indicated by the increase in the yellow bars) with a concomitant
decrease in the level of wild-type embryos (blue bars). A number of embryos
(indicated by the red bars) display a phenotype intermediate between wild type
and mutant, characterized by a less dramatic dilation of the heart and some
with weak circulation. heg, heg exon 11 donor morpholino injected;
san, san exon 1 donor morpholino injected; vtn, vtn exon 2
morpholino injected.
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