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Fig. 6. Infection with RCAS:Lef1-En causes a reduction in Collagen IX in the
iris epithelium. (A,B) ISH for Collagen IX at E4.5 from eyes
infected with RCAS:Lef1-En. Note only the area with higher infection [stronger
-gag (3C2) staining at the peripheral tip of the retina in A' compared
with B'] shows a reduction of Collagen IX expression (A); weaker infection of
RCAS:Lef1-En does not affect expression (B). (C-H) Effects of sustained
expression of Lef1-En on iris development. (C) A normal eye after removal of
the cornea at E14 is shown at the top and an example of an eye infected with
RCAS:Lef1-En is shown at the bottom. The arrow points to an area with a defect
in the anterior structures, particularly the iris. (D-F) H&E staining on
sections from an eye partially infected with RCAS:Lef1-En reveals a slightly
disorganized pars plicata and a severe reduction in the iris on the left side
(arrowhead, E), compared with the unaffected normal iris (arrow, F). (G,H) IHC
of -SMA (red) in stroma of the affected (arrowhead, G) compared with
the non-affected (arrow, H) areas of the iris. Nuclear counter staining was
done with DAPI (blue). (I,J) Hypopigmentation of RPE induced by
RCAS:Lef1-En at E14.5. Compared with a control RCAS:GFP-infected eye (left),
an eye from a RCAS:Lef1-En-infected animal (right) shows patches with less
pigmentation (arrows). H&E staining of the infected eye shows RPE areas
with partial pigmentation defects (arrows). Le, lens. Scale bar: 3 mm.
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