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Figure 5


Fig. 5. Timing of sax-8 action. (A) nu319ts animals grown at 15°C are wild type for chemosensory amphid neuron position (in blue), but are mutant by the fourth larval and adult stages when grown at 25°C (in red). Wild-type animals are fully normal at all three temperatures, and dig-1(nu345), a non-temperature-sensitive mutant displays the same penetrance at all temperatures (data not shown). (B) The adult phenotype of nu139ts animals does not depend on the temperature at which they underwent the development of the nervous system (in embryos and early L1 larvae), but rather on the temperature to which they were exposed as L2, L3, L4 larvae and adults. Animals grown at 15°C up to a given developmental stage, shifted to 25°C and scored as 3-day-old adults are shown in red. Animals grown at 25°C up to a given developmental stage, shifted to 15°C and scored as 3-day-old adults are shown in blue. L1, L2, L3 and L4, first, second, third, and fourth larval stage, respectively; YA, young adult; A, 3-day-old adult. The blue and red broken lines give the penetrance for adult mutants grown continuously at 15°C and 25°C, respectively. Error bars indicate the standard error of proportion. n=50-250 for each data point.





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