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Fig. 7. dig-1 acts non-autonomously to maintain axon position in the
VNC and the lateral ganglion in the head. (A) Expression pattern of
dig-1 gfp reporter constructs (see Fig. S2 in the supplementary
material for details on reporter constructs and transgenic lines examined).
Expression in the gut and in non-neuronal head cells in the commastage embryo
(i,ii). Expression in non-neuronal head cells, as well as in body muscles, in
late threefold embryos (iii). Expression in the hypodermal syncytium hyp7 in
larvae and adults (iv). Strong expression in head muscles and other mesodermal
cells, including GLRs, the head mesodermal cell (hmc) (v), and in all the body
wall muscles and coelomocytes (vi, vii). The shape of a single body wall
muscle cell is outlined in vi. Expression remained strong throughout larval
and adult stages. Scale bars: 10 µm. (B) Mosaic analysis. Rescuing
cosmids K07E12 and R05H11 were injected at 
15 ng/µl into
dig-1(ky188);oyIs14 animals together with myo-3::gfp and
F25B3.3::DsRed as lineage markers. One out of 4 of the transgenic
lines rescued the PVQ defects and was used for mosaic analysis. Two classes of
animals were scored for rescue of axonal defects. `P1-' animals in which the
AB cell descendants express F25B3.3::DsRed pan-neuronally, including
the ttx-3::gfp-expressing AIY neurons, and myo-3::gfp in a
limited number of muscle cells, but not in the body muscle cells. Second,
`AB-' animals in which body wall muscle cells, but not enteric muscles,
express gfp and in which the neurons do not express
F25B3.3::DsRed. (C) Scoring of mosaic, as well as of the
transgenic and non-transgenic control animals.
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