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Figure 1


Fig. 1. cpa or cpb mutant clones are extruded from the wing blade epithelium. All panels show third instar wing discs. (A-C) Standard confocal sections. (A) cpa107E, (B) tsr110M and (C) captE636 mutant clones are marked by the absence of GFP (green). (D-H) Optical cross-sections through the wing disc epithelium. Mutant clones are positively labeled with GFP (green) and sections are stained with anti-Dlg (red) and anti-Arm (blue) to outline apical cell membranes. (D) cpa107E; (E) cpbM143; (F) tsr110M; (G) captE593; (H) cpa107E mutant clones overexpressing full-length cpa. cpa or cpb mutant clones are extruded basally in the wing blade primordium (red arrows), but not the notum; this extrusion is rescued by full-length cpa. tsr and capt mutant clones are not extruded. The white arrows define the wing blade region. Dorsal is towards the left on optical cross-sections in this and subsequent figures.





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