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Fig. 5. cpa is required in vestigial expressing cells. (A-G)
Third instar wing imaginal discs. (A-A'') Standard confocal sections of
wing discs with cpa mutant clones marked by the absence of GFP (green
in A,A'') and stained with anti-Vg (magenta in A',A''). Vg is
expressed in cpa mutant clones. (B-G) Discs in which clones are
positively labeled with GFP (green) and stained with anti-Dlg (blue) and
anti-Caspase 3 (red). (B-D) Standard confocal sections; (E-G) optical cross
sections. (B,E) cpa43D mutant clones are extruded and die
in the wing blade region; (C,F) clones overexpressing vg are not
extruded and survive; (D,G) cpa43D mutant clones
overexpressing vg are extruded and die in all regions of the disc.
Red arrows in E,G indicate clones within the notum primordium. The white
arrows define the wing blade region.
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