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Figure 1


Fig. 1. Slug is an unstable protein. (A) Embryos were injected with mRNA encoding either Slug or Id3, allowed to develop to stage 8 and treated with cycloheximide (CHX) to prevent further protein synthesis. Western blot analysis demonstrates that Slug protein is highly unstable and is regulated in a time frame similar to that of Id3. (B) The instability of Snail family members is evolutionarily conserved. Western blot showing that in contrast to Xl Sox10, expression of Xl Slug, Xl Snail, Mm Snail and Dm Snail is greatly diminished or undetectable by migrating neural crest stages. All blots were stripped and re-probed with actin as a loading control. Representative actin shown is for Slug blot. (C) Embryos injected with mRNA encoding either Sox10 or Slug were treated with CHX at stage 8. Western blot analysis demonstrates that Sox10 has a significantly longer half-life than Slug. (D) Embryos co-injected with DNGSK3ß and either ß-catenin, Slug or Snail were treated with CHX at stage 8 and collected at the time points indicated. Whereas ß-catenin was robustly stabilized by the presence of DNGSK3ß, Snail was only modestly stabilized and Slug stability was not affected. Actin was used as a control.





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