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Figure 2


Fig. 2. Slug stability is positively regulated by activation of the neural crest regulatory program. (A-F) Embryos were co-injected with Slug and either FoxD3 (A), Sox9 (B), Twist (C), Opl (D), Smad1 (E) or Snail (F) and treated with CHX at stage 8 to prevent further protein synthesis. Samples collected at the time points indicated were subjected to western blot analysis. FoxD3, Sox9, and Twist all increased Slug stability, whereas Smad1, Snail and Opl were unable to do so. Actin was used as a control.





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