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Figure 5


Fig. 5. sage is directly regulated by Fkh and Sage. (A) 1.2 kb of sequence upstream and spanning the translation start site (green letters) of sage is sufficient to direct Fkh-dependent salivary gland expression of the lacZ reporter gene. This sequence contains five consensus bHLH-binding sites (red circles and sequences) and one Fkh-binding site (blue oval and sequence). (B,C) The wild-type Sage 1.2-lacZ construct gave robust salivary gland expression both early and late. (D,E) Expression of the wild-type Sage 1.2-lacZ was diminished in fkh mutant salivary glands, which do not invaginate. (F,G) Mutations disrupting either the Fkh site or (H,I) the bHLH sites resulted in reduced salivary gland expression of ßgal. (J,K) Loss of both Fkh and bHLH sites resulted in reduced expression of the reporter gene to levels observed in fkh mutants (compare salivary gland expression of ßgal in J and K with D and E).





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