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Fig. 5. miR156 is responsible for the temporal expression of
SPL3. (A) Blots of total RNA from the shoot apex of plants
grown for different lengths of time in SD. The approximate number of leaves
greater than 100 µm in length present at each time point is indicated in
parentheses. Actin was used as a loading control for SPL3, and
U6 was used as a loading control for miR156. (B) GUS
expression in transgenic plants of different ages. Leaf number 7 is indicated.
The approximate number of leaves greater than 100 µm in length in these
samples is indicated in parentheses. (C) Quantitative analysis of GUS
activity in extracts of the lines illustrated in B. Error bars indicate s.e.m.
(D) Northern analysis of miR156 levels in the transgenic lines
illustrated in B and C.
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