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Figure 4


Fig. 4. Endocardial Gata4 activity is required for mesenchyme formation in AV cushion explants. (A,B) Gata4T2del AVC explants produced markedly fewer mesenchymal cells than control explants. (C) Quantitation of the number of cells with mesenchymal morphology from control and Gata4T2del explants (n=3). (D,E) Failure of endocardial activation in Gata4T2del AV explant cultures. In control embryos, Pecam-expressing endothelial cells lost cell-cell contacts and adopted a rounded shape (arrow). Some rounded cells expressed SMA (white arrowhead) and acquired a mesenchymal morphology (yellow arrowhead). In mutant explants, endothelial cells remained in a sheet (star) and did not adopt a rounded shape. No mesenchymal cells or cells positive for both Pecam and SMA were detected (n=4). Myocardium (M) of control and mutant explants stained positive for SMA.





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