|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 8. AV valve remodeling requires Gata4 expression in endocardium and its
derivatives. Gata4T2del/Ki
(Gata4Ki/flox; Tie2Cre+) embryos are deficient
for Gata4-Fog interaction in the endocardium and its derivatives. (A-D)
AV cushions were normally populated by mesenchymal cells at E12.5 (asterisks,
C,D), indicating that the Gata4-Fog interaction is not essential for EMT. The
liver (L) was normal in size and morphology. Boxed regions of A,B are enlarged
in C,D. (E,F) A common atrioventricular canal (asterisk) was
present in all Gata4T2del/Ki embryos at E14.5, whereas in
control embryos the AVC was septated by the fusion of the superior and
inferior cushions. (G) Decreased proliferation of cushion mesenchyme in
Gata4T2del/Ki embryos. Proliferation was measured as the
percentage of nuclei that stained positive for BrdU (red). Nuclei were stained
with DAPI. Myocyctes were labeled with desmin (green). Three sections from
three different E12.5 embryos were examined per group by a blinded observer
(*P<0.05).
|
