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Fig. 2. Correct patterning of ectopic somites and improper patterning of LPM in
Bmpr-MORE embryos. (A-F') E8.5. Upper side is
anterior. Dorsal or ventral view is shown for control or Bmpr-MORE
embryos, respectively. Uncx4.1, a caudal marker of each somite, was
expressed in a column on each side of control embryos (A, arrowheads). Three
or more irregular columns of somites occurred and expressed Uncx4.1
on each side of mutant embryos (A', arrowheads). Double in situ analyses
for Uncx4.1 (black) and Mox1 (yellow) (B,B').
Mox1, a marker of somites, was expressed in the entire region of each
somite (red arrow) and Uncx4.1 was expressed in the caudal part of
each somite in both control and mutant embryos (black arrow). The bracket
shows one somite. Epha4 is a rostral presomitic mesoderm marker (C,
arrowheads). The expression domain was expanded in Bmpr-MORE embryos
(C', arrowheads). Mox1 was expressed in somites (D,E, arrows)
but not in LPM in control embryos. In mutants, expression of Mox1 was
observed in somites (D',E', arrows) and in LPM (D',E',
red arrowheads, see inset). (E,E') Sections of D,D'. Pax1 is
expressed in sclerotome in control embryos (F, arrowheads). Pax1 is
expressed in expanded somites of Bmpr-MORE embryos (F',
arrowheads). (G-I') Expression of LPM markers. (G,G')
Ventral view at E8.5. Upper side is anterior. Foxf1 was expressed in
LPM of control embryos (G, arrow). The expression of Foxf1 was patchy
and weak in Bmpr-MORE embryos (G', arrows). (H-I')
Posterior view. In control embryos, Bmp4 was expressed in LPM
(arrow), proximal primitive streak (black arrowhead) and allantois (red
arrowhead) (H). Bmp4 was not expressed in mutant LPM (H',
arrow). Black and red arrowheads show the posterior primitive streak and
allantois, respectively (H'). The expression of Lim1
(I,I', arrowhead) in migrating LPM. The Lim1 was not expressed
(I') or was decreased in mutant embryos. Scale bars: 300 µm for
A-A',C,C',D,D',F,F'; 600 µm for B,B'; 250
µm for G-H'; 125 µm for I,I',E,E'.
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