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Fig. 3. Recruitment of prospective PXM is delayed during gastrulation in
Bmpr-MORE embryos. (A,A') No allantoic bud
stage. Brachyury was expressed in the primitive streak (A, arrow), node and
notochord of control embryos. The rostral end of the notochord was at the
anterior of the embryo (A, arrowhead). The expression of Brachyury
did not extend to the anterior in Bmpr-MORE embryos (A', arrow
and arrowhead). (B-C') The early to late allantoic bud stage.
Foxa2 was expressed in the node (B, lower arrowhead) and notochord of
control embryos. Upper arrowheads indicate embryonic/extra-embryonic border.
As the node is formed at the distal region of the primitive streak, the length
of the primitive streak corresponds to the length between two arrowheads. The
length of the primitive streak of Bmpr-MORE embryos was shorter
compared with that of control embryos (B', arrowheads). Definitive
endoderm develops in the mutant embryo (B', arrow). (C,C') Noggin
was expressed in the node (lower arrowheads). The length of the primitive
streak was shorter in mutant embryos (distance between arrowheads).
(D,D') The head-fold stage. (D) Tbx6 was expressed in
the mesoderm except axial mesoderm (between arrowheads). (D') The length
of the primitive streak appeared to be similar between control and
Bmpr-MORE embryos (distance between arrowheads). (E,E')
The late streak stage. Lefty2 was expressed in LPM and PXM (between
arrowheads). (E) In Bmpr-MORE, Lefty2 expression was decreased
(E', between arrowheads). (F-G') The early to late allantoic bud
stage. Lefty2 was expressed in migrating LPM and PXM (F, black and
red arrowheads, respectively). In Bmpr-MORE, the migrating LPM
expressed Lefty2 (F', black arrowhead). More recently recruited
mesoderm expressing Lefty2 was observed at the middle and distal
regions of the primitive streak (F', red arrow and arrowhead,
respectively). Pcdh8 (PAPC on figure) was also expressed in LPM and
PXM (G, black and red arrowheads, respectively). In Bmpr-MORE, the
migrating LPM expressed Pcdh8 (G', black arrowhead). More
recently recruited mesoderm expressing Pcdh8 was observed at middle
and distal regions of the primitive streak (G', red arrow and arrowhead,
respectively). Lefty2 was not expressed at the head-fold stage in
control embryos (H). In Bmpr-MORE embryos, Lefty2 was
still strongly expressed at distal and middle embryonic region (H',
arrowhead and arrow, respectively). Scale bars: 100 µm for
A,A',E,E'; 250 µm for B-D'; 200 µm for F-H'.
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