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Fig. 5. Inhibition of FGF signaling by a specific antagonist for FGFR1 in
Bmpr-MORE embryos. (A) Control embryos developed to the
late streak to allantoic bud stage. Bmpr-MORE embryos showed a poorly
developed amniotic fold (red arrowheads) and less extended primitive streak
(yellow arrows) in culture, as was observed in vivo. Inset shows genotyping
results for these embryos. Embryos positive for Cre and heterozygous
for Bmpr1a-null allele are Bmpr-MORE embryos (inset, pink
stars). (B) Control embryos developed to the allantoic bud stage.
Bmpr-MORE embryos developed amnion, chorion and allantois (red
arrowheads), and a well-extended primitive streak (yellow arrow). Embryos
positive for Cre and heterozygous for Bmpr1a-null allele are
Bmpr-MORE embryos (inset, pink star). (C-F) Cultured embryos
stained with phospho-Erk1/2 antibody. (C) Control embryos cultured with DMSO.
Ectoplacental cone (EPC) (arrow) and chorion (arrowheads) were strongly
stained. (D) Mutant embryos cultured with DMSO. Ectoplacental cone (arrow) and
the amniotic fold (orange arrowhead) showed strong expression. The
extra-embryonic ectoderm in the ectoplacental cavity was weakly stained (black
arrowheads). (E) Control embryos cultured with 20 µM SU5402. Expression in
the ectoplacental cone was not affected (arrow), but that of the chorion was
significantly decreased. (F) Mutant embryos cultured with 20 µM SU5402.
Expression in the ectoplacental cone was not affected (arrow) but that of the
chorion was significantly decreased (arrowheads). Scale bars: 500 µm for
A,B; 100 µm for C-F.
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