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Fig. 3. Sox8-depleted embryos fail to express Sox10 at the neural plate
border. (A) Increasing amounts of Sox8mo (10 ng, 100 ng and 1000
ng) blocks translation directed by Sox8 mRNA. The same morpholino (500 ng)
fails to block Sox9 and Sox10 translation. (B) Embryos injected in one
blastomere at the two-cell stage with 30 ng of Sox8mo exhibit reduced Sox10
expression at stage 17, while Slug, Sox9 and Sox2 expression appears largely
unaffected at this stage. (C) Quantification of the in situ
hybridization results. The numbers in parenthesis indicate the number of
embryos analyzed. (D) Sox8mo (500 ng) does not interfere with
translation of a mutated Sox8 mRNA (mSox8). (E) Rescue experiments were
performed by injection of an animal dorsal blastomere at the eight-cell stage.
Bilateral Sox10 expression is rescued in Sox8mo-injected embryo by
co-injection of mSox8 mRNA (Sox8mo+mSox8). Single injection of mSox8 expanded
the Sox10 expression domain. Injection of a 5 bp mis-matched Sox8 morpholino
(Sox8mis) had no effect on Sox10 expression. (F) Quantification of the
in situ hybridization results. The numbers in parentheses indicate the number
of embryos analyzed. (B,E) Dorsal view, anterior is towards the top. RNA
encoding the lineage tracer ß-galactosidase was co-injected to identify
the injected side (red staining) (left side in B and right side in E).
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