|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Caudal hindbrain possesses a graded nonpermissive/repulsive activity for
the cC-VC axons. (A,B) Two types of grafting experiments.
The positions of the grafts were approximately 1.2 mm (A) and 1.8 mm (B)
caudal to gVIII, respectively. Almost all cC-VC axons stalled or turned away
before entering the graft in B (n=12), whereas a small proportion of
the cC-VC axons still grew through the graft in A. (C) A piece of
rostral hindbrain along the cC-VC path was cut and put back as control.
Majority of cC-VC axons could grow through the graft (n=10). All
grafts were soaked in DiO before transplanting into the hosts, thus the
borders of the grafts were discerned by DiO signal combined with bright-field
illumination. DiO signal was displayed only in C, but removed for better
illustration of axons in the grafts in other images. White dots outline the
border of grafts in A-C. (D) Quantification of the percentage of axons
entering the graft (*P<0.0001, Mann-Whitney U
test) (error bar indicates s.d.). Scale bar: 400 µm.
|
