|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Impaired chemoattractant response of ENCCs in RET S697A mutant mice.
(A) Gut segments were dissected out from wildtype and homozygous RET
S697A mutant mice at E12.5, and cultured on collagen gels with GDNF-soaked
beads (dotted circles). After 4 days of culture, immunostaining with
anti-PGP9.5 antibody was carried out to visualize ENCCs. Bars, 500 µm.
(B) PGP9.5-positive area was measured in the quadrant facing
GDNF-soaked beads (towards) and in the opposite quadrant (away). Six explants
for each experiment. *P<0.01 compared with the distal
quadrant. He, heterozygotes. (C) Lamellipodia formation in migrating
ENCCs. F-actin was visualized with Alexa-phalloidin staining. Arrowheads
indicate lamellipodia formation. Scale bars: 20 µm. (D) Quantitative
analysis of lamellipodia formation. The number of lamellipodia was counted in
the migrating cell front in the proximal and distal area. The number of
lamellipodia per cell surface length (1 mm) was calculated. Six explants for
each experiment. *P<0.01 compared with the distal
quadrant.
|
