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Fig. 2. Loss of mesDA neurons in postnatal Ngn2 mutant mice. (A-M) In
postnatal Ngn2 mutants, the number of Th-positive neurons was
markedly reduced in both the SN and the VTA (A,B,D,E), and a population of
Th-positive cells located close to the midbrain aqueduct was completely
missing (F,G). Parallel sections stained for Vmat2 showed that no other cells
present in the SN-VTA area displayed a DA phenotype (H,I). The Th-positive
innervation of striatal and limbic forebrain areas was normal, although the
target areas were reduced in size (L,M). The Ngn2 mutants were
similar in size to wild-type mice at birth but showed a reduced weight gain
postnatally (C), and the size of their brain was reduced by about 20% (J,K).
Stars indicate the rostrocaudal levels used for cell counting. (N,O)
Cell counts at P0 and P18 revealed a more than 50% loss of Th-positive neurons
in the SN and VTA of the Ngn2 mutants. *P
0.01
relative to wild type (N), and in wild type adn heterozygous (O) (Student's
t-test). Scale bars: 500 µm.
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