spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Deletion of floxed Myog sequences using CMV-cre and analysis of embryonic skeletal muscle at E18.5. (A) Southern genome hybridization of yolk-sac DNA digested with EcoRI from embryos resulting from a Myogflox/+;CMV-cre/+ intercross and probed with Myog cDNA. Deletion of the floxed myogenin sequences produced a 3 kb EcoRI fragment representing the Myogflox{Delta} allele. The 4 kb EcoRI fragment represents the wild-type allele (Myog+). Lanes 1 and 2 show DNA from Myogflox{Delta}/flox{Delta} embryos; lanes 3 and 4 show Myogflox{Delta}/+ embryos; and lanes 5 and 6 show wild-type embryos. (B) Diaphragms of E18.5 Myog+/-, Myog-/- and Myogflox{Delta}/flox{Delta} embryos. The genotype is shown above each image. Scale bar: 100 µm. (C) Hindlimb sections from E18.5 Myogflox{Delta}/+ and Myogflox{Delta}/flox{Delta} embryos immunostained with polyclonal M225 anti-myogenin antibody. Arrows indicate positively stained cells. Scale bar: 100 µm.





Right arrow Return to article