DEV02235 Supplementary Material

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• Supplemental Figure 1 -

  Fig. S1. The sperm defect caused by loss of uri-1 function depends on proliferation. DAPI staining of glp-1; uri-1(RNAi)F1 and uri-1(RNAi)F1 sperm. Loss of uri-1 by RNAi in the glp-1 mutant suppresses the URI-1 sperm phenotype leading to the glp-1 phenotype (premature meiotic entry), without any visible defect in spermatogenesis, indicating that the process of spermatogenesis per se is not affected through the loss of URI-1 function. As uri-1(lf) leads to proliferation defects that seem to have an impact on spermatogenesis in the URI-1 depleted animal (as shown above), we conclude that uri-1 is indirectly required for sperm differentiation and meiotic cell cycle. Arrows indicate haploid sperm and arrowheads indicate spermatids. For all the pictures, anterior is towards the left and dorsal towards the top. Scale bar: 10 mm.