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Fig. 7. Forced Runx2 expression rescues an Nkx3.2-induced but not a
PTHrP-induced blockade of chondrocyte maturation. (A) Forced Runx2
expression rescues an Nkx3.2-induced blockade of chondrocyte maturation in
vivo. The right wing buds of E3 chick embryos were co-infected with
RCAS(A)-Nkx3.2 plus RCAS(B)-Alkaline Phosphatase (AP) viruses (b,e,h,k,n), or
RCAS(A)-Nkx3.2 plus RCAS(B)-Runx2 viruses (c,f,i,l,o), and reincubated until
E10. The control contralateral uninfected wing (a,d,g,j,m) and the co-infected
wings were analyzed either by Alcian Blue/Alizarin Red staining (AB/AR, a-c),
or by section ISH with the indicated probes (d-o). (B) Nkx3.2 does not
block chondrocyte hypertrophy induced by ectopic Runx2 in vitro. Explants of
presomitic mesoderm were co-infected with the indicated viruses and cultured
sequentially in Shh for 2 days and Bmp4 for 3 days. Gene expression was
assayed by RT-PCR. (C) PTHrP and forskolin can block chondrocyte
hypertrophy induced by ectopic Runx2 in vitro. Explants of presomitic mesoderm
were infected with RCAS(A)-Runx2 and cultured as described in part B, in
either the absence (lanes 1 and 5) or in the presence of increasing amounts of
either PTHrP (lanes 2-4) or forskolin (lanes 6 and 7), as indicated. Gene
expression was assayed by RT-PCR.
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