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Fig. 4. Different regions of ectoderm are differentially responsive to Fgf2.
(A) Ectoderm was taken from the anterior epiblast of HH stage
3+-4 chick embryos, or from the presumptive trigeminal, or
presumptive otic ectoderm, or lateral to the presumptive trigeminal ectoderm
of 0-4 ss embryos. Cultures were grown in 50 ng/ml Fgf2 for 24 hours.
(B) Pax2 is expressed in presumptive otic or presumptive trigeminal
level ectoderm, but not ectoderm taken from lateral regions or from the
anterior epiblast. (C) Histogram showing Pax2 induction in different
ectoderm populations in the presence or absence of Fgf2. Numbers above the
columns indicate the number of explants tested in each case. (D)
Sections of 2 ss chick embryos through the region of the presumptive
trigeminal placode. Embryos were processed with an in situ probe for
Eya2, or with an antibody that recognizes all Distalless proteins
(pan-Dll). Expression of pre-placodal markers is seen in ectoderm adjacent to
the neural plate (black arrow or black and white brackets), but not in lateral
ectoderm (red arrow and brackets).
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