spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Pre-placodal markers are upregulated following grafting adjacent to the anterior-most neural plate, but not the trunk neural plate. (A) Anterior epiblast was isolated from HH stage 3+-4 quail embryos and grafted into chick hosts of between 0-4 ss at the level of either the most anterior part of the pre-placodal region (at the level of the presumptive nasal and lens placodes) or adjacent to the neural plate in the trunk (posterior to the pre-placodal region). Embryos were examined after 8 hours with a pan-Distalless antibody (pan-Dll) or an Eya2 probe, together with QCPN antibody to label quail tissue. Grafts adjacent to the anterior neural plate express both markers, but grafts adjacent to trunk level neural plate - which lie posterior to the pre-placodal region - do not. (B) Trunk ectoderm from young (0-2 ss) or old (7-9 ss) quail embryos was grafted into the pre-placodal region of 0-4 ss chick hosts. Embryos were examined after 8 hours with a pan-Distalless antibody (pan-Dll) or an Eya2 probe, together with QCPN antibody to label quail tissue. Young trunk ectoderm expresses both markers, but older ectoderm does not.





Right arrow Return to article